MALDI Mass Spec Imaging

Use of PNGase F PRIME™ in MALDI-Imaging of Glycans from Clinical Tumor Bearing Tissue Samples

Advantages of Ion Mobility for MALDI Imaging of N-glycans from Tumor Bearing FFPE Tissue in Clinical Research
Goal: Describe the separation of N-glycans Isobaric species by Ion Mobility directly ionized from a Formalin-Fixed Paraffin-Embedded (FFPE) tissue section showing different localizations within the tumor and non-tumor tissue types for clinical research.
Waters Technology Brief - Ion Mobility f[...]
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Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a unique and well developed tool for probing the protein content of formalin-fixed, paraffin-embedded tissue (FFPE). Integral to this approach is the application of trypsin, and more recently peptide N-glycosidase F, to release tryptic peptides or N-glycans from tissue and report localization of distinct species. This is typically done on serial or adjacent tissue sections, and there is an emerging need to understand the co-localized protein population linked to the exact same regions of N-glycans.

This manuscript describes an approach where N-glycans are first imaged from a tissue section followed by reprocessing of the same tissue section for tryptic peptide MALDI IMS. It also describes strategies for co-localizing peptides to target N-glycans or N-glycan regions.

 

This manuscript was authored by Peggi M. Angel, Anand S. Mehta, Kim Norris-Caneda, and Richard R. Drake, all from the Medical University of South Carolina, and was first published in final edited form as: Methods Mol Biol. 2018; 1788:225-241.

 

doi:10.1007/7651_81 and is available in PMC (PubMed Central) 2019 January 01.

This work was supported by the National Institutes of Health/National Cancer Institute R21 CA185799 to Richard R. Drake.

 

MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section
MALDI MSI Protocols.pdf
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