Sialidase PRIME-LY™
| Target Core | Asparagine-linked (N-linked) or serine/threonine linked (O-linked) oligosaccharides (complex/terminally sialyated) |
|---|---|
| Compatible Glycoprotein | Native and denatured |
| Source of Sialidase PRIME-LY™ | recombinant, expressed in E. coli. |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 Unit of enzyme will completely de-sialate 10 µg of denatured alpha 1 anti-trypsin following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Quantity | Each standard vial of lyophilized format enzyme contains 100µg of Sialidase PRIME™ lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of cell culture grade water used in the reconstitution process. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. Sialidase PRIME-LY™ contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |