|
|
| Compatible Glycoprotein |
Native and denatured |
| Source of PNGase F PRIME-LY™ |
Recombinant, E. coli |
| Total Protocol Length |
30 minutes to 24 hours |
| Typical Yield |
1 Unit of enzyme will completely deglycosylate 10 µg of denatured RNase B following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Quantity |
Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (2mg.mL)] lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process. |
| Purity |
≥95% as determined by SDS PAGE |
| Contains Glycerol |
No |
| Compatible with Mass Spec |
Yes |
| Affinity tag for purifcation |
Yes. PNGase F PRIME-LY™ contains a His-tag. |
| Reaction Conditions |
In vitro and in situ |
| Storage |
-20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life |
1 year |
| Microbe Free |
Yes, lot tested |