PNGase F PRIME-LY™

PNGase F PRIME-LY™

Target Core asparagine-linked (N-linked) oligosaccharides (mannose, hybrid, and complex)
Compatible Glycoprotein Native and denatured
Source of PNGase F PRIME-LY™ Recombinant, E. coli
Total Protocol Length 30 minutes to 24 hours
Typical Yield 1 Unit of enzyme will completely deglycosylate 10 µg of denatured RNase B following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit.
Quantity Each standard vial of lyophilized format enzyme contains 100 µg of PNGase F PRIME™ [1,000,000 Units/mL (2mg.mL)] lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of dH2O used in the reconstitution process.
Purity ≥95% as determined by SDS PAGE
Contains Glycerol No
Compatible with Mass Spec Yes
Affinity tag for purifcation Yes. PNGase F PRIME-LY™ contains a His-tag.
Reaction Conditions In vitro and in situ
Storage -20°C or 4°C (avoid multiple freeze-thaws)
Shelf Life 1 year
Microbe Free Yes, lot tested
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