The following is a Partial List of Publications citing the use of PNGase F PRIME™:



Powers, T. W. et al. Matrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues. Analytical Chemistry 85, 9799-9806. PMID: 24050758. (2013).


Powers, T. W. et al. MALDI imaging mass spectrometry profiling of N-glycans in formalin-fixed paraffin embedded clinical tissue blocks and tissue microarrays. PLoS One 9, e106255, pp106251-106211. PMID: 25184632 (2014).





Heijs B, Potthoff A, Soltwisch J, Dreisewerd K. MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.  Anal. Chem. 2020,92, 13904-13911.


Boyaval F, Van Zeijl R, Dalebout H, Holst S, van Pelt GW, Fariña- Sarasqueta A, Mesker WE, Tollenaar R, Morreau H, Wuhrer M, Heijs B. N-glycomic signature of stage II colorectal cancer and its association with the tumor microenvironment. Mol Cell Proteomics 2020 Oct 20:mcp.RA120.002215. doi: 10.1074/mcp.RA120.002215.


Biswas, B., Batista, F., Akintayo, A., Aguilan, J. & Stanley, P. Transgenic Rescue of Spermatogenesis in Males With Mgat1 Deleted in Germ Cells. Frontiers in Cell and Developmental Biology 8, 212 (2020). PMCID: PMC7142241.


Scott, D. A. et al. Specific N-Linked glycosylation patterns in areas of necrosis in tumor tissues. International Journal of Mass Spectrometry 437, 69-76 (2019). PMCID: PMC6483403.


Briggs, M. T. et al. MALDI Mass Spectrometry Imaging of Earlyand LateStage Serous Ovarian Cancer Tissue Reveals StageSpecific NGlycans. Proteomics 19, e1800482 (2019). PMID: 31364262.


West, C. A. et al. N-Linked glycan branching and fucosylation are increased directly in hepatocellular carcinoma tissue as determined through in situ glycan imaging. Journal of Proteome Research 17, 3454-3462. (2018). PMID: 30110170.


Drake, R. R., West, C. A., Mehta, A. S. & Angel, P. M. MALDI mass spectrometry imaging of N-Linked glycans in tissues. Advances in Experimental Medicine and Biology 1104, 59-76 (2018). PMID: 30484244.


Drake, R. R. et al. MALDI Mass Spectrometry Imaging of N-Linked Glycans in Cancer Tissues. Advances in Cancer Research 134, 85-116 (2017). PMCID: PMC6020705. This review article contains several images of PNGase F Prime used for MALDI IMS


Holst, S. et al. Linkage-specific in-situ sialic acid derivatization for N-Glycan Mass Spectrometry Imaging of FFPE tissues. Analytical Chemistry 88, 5904-5913 (2016). PMID: I27145236.


Everest-Dass, A. V. et al. N-Glycan MALDI imaging mass spectrometry on formalin-fixed paraffin-embedded tissue enables the delineation of ovarian cancer tissues. Molecular & Cellular Proteomics 15, 3003-3016. PMCID: PMC5013313. (2016).





Angel, P. M. et al. Zonal Regulation of Collagen Types and Post-Translational Modifications In Prostatic Benign and Cancer Tissues By Imaging Mass Spectrometry. The Prostate In press. (2020).


Clift, C. L., Mehta, A. S., Drake, R. R. & Angel, P. M. Multiplexed Imaging Mass Spectrometry of Histological Staining, N-Glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research. Methods in Molecular Biology In press (2019).


Angel, P. M. et al. Advances in MALDI Imaging Mass Spectrometry of Proteins in Cardiac Tissue, Including the Heart Valve. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1865, 927-935. PMCID: PMC5527275. (2017).





Stanback Alexandra E, Conroy Lindsey R, Young Lyndsay EA, Clarke Harrison A, Allison Derek B, Sun Ramon C.  Protocol - Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging. STAR Protocols 2, 100304 March 19, 2021.


Voruganti S, Xu J, Li X, Balakrishnan G, Singh SM, Kar SR, Das TK. A Detailed Protocol for Generation of Therapeutic Antibodies with Galactosylated Glycovariants at Laboratory Scale Using In-Vitro Glycoengineering Technology. J Pharm Sci. 2020 Oct 8:S0022-3549(20)30590-6.

Cites Industrial use of PNGase F PRIME™ to QC therapeutic antibodies.


Angel, P. M., Mehta, A. S. & Drake, R. R. Array-based N-Glycan Profiling of Cells in Culture. Methods in Molecular Biology in press (2020).


Black, A. P. et al. A novel mass spectrometry platform for multiplexed N-Glycoprotein biomarker discovery from patient biofluids by antibody panel-based N-Glycan imaging. Analytical Chemistry 91, 8429-8435 (2019).

Cites the use of PNGase F PRIME™ in multiplexed analysis of N-glycoproteins from biofluids.

Black, A. P., Angel, P. M., Drake, R. R. & Mehta, A. S. Antibody Panel Based NGlycan Imaging for NGlycoprotein Biomarker Discovery. Current Protocols in Protein Science 98, e99 (2019).


Angel, P. M. et al. A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells. Journal of Proteome Research 18, 3630-3639. (2019).


Heijs, B. et al. Multimodal mass spectrometry imaging of N-glycans and proteins from the same tissue section. Analytical Chemistry 88, 7745-7753.PMID: 27373711 (2016).


Drake, R. R., Powers, T. W., NorrisCaneda, K., Mehta, A. S. & Angel, P. M. In Situ Imaging of NGlycans by MALDI Imaging Mass Spectrometry of Fresh or FormalinFixed ParaffinEmbedded Tissue. Current Protocols in Protein Science 94, e68. PMID: 30074304. (2018).


Angel, P. M., Mehta, A., Norris-Caneda, K. & Drake, R. R. in Methods in Molecular Biology: Tissue Proteomics Vol. MALDI Imaging Mass Spectrometry of N-Glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section. doi: 10.1007/7651_2017_81. PMID: 29058228 (Humana Press, 2017).





West, C. A., Drake, R. R. & Mehta, A. S. Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry. Journal of Proteome Research in press. (2020) Jun 8. doi: 10.1021/acs.jproteome.0c00024. Online ahead of print.





Takeda Y, Jamsransuren D, Matsuda S, Crea R, Ogawa H. The SARS-CoV-2-Inactivating Activity of Hydroxytyrosol-Rich Aqueous Olive Pulp Extract (HIDROX®) and Its Use as a Virucidal Cream for Topical Application. Viruses 2021, 13, 232.


Biswas KB, Takahashi A, Mizutani Y, Takayama S, Ishitsuka A, Yang F, Iddamalgoda A, Katayama I, Inoue S. GPNMB is expressed in Human Epidermal Keratinocytes but disappears in the Vitiligo Lesional skin. Sci Rep. 2020 Mar 18:10(1):4930. doi: 10.1038/s41598-020-61931-1.


Esteban PF, Garcia-Ovejero D, Paniagua-Torija B, Moreno-Luna R, Arredondo LF, Zimmer A, Martín ÁA, Molina-Holgado E. Revisiting CB1 cannabinoid receptor detection and the exploration of its interacting partners. J Neurosci Methods. 2020 May 1;337:108680. doi: 10.1016/ j-jneumeth.2020.108680. Epub Mar 4. PMID: 32145227.


Drake RR, McDowell C, West C, David F, Powers TW, Nowling T, Bruner E, Mehta AS, Angel PM, Marlow LA, Tun HW, Copland JA. Defining the human kidney N-Glycome in normal and cancer tissues using MALDI imaging mass spectrometry. J Mass Spectrom. 2019 Dec 20.


Watanabe K, Watanabe K, Watanabe Y, Fujioka D, Nakamura T, Nakamura K, Obata JE, Kugiyama K. Human soluble phospholipase A2 receptor is an inhibitor of the integrin-mediated cell migratory response to collagen-I.  Am J Physiol Cell Physiol. 2018 Sep 1;315(3):C398-C408. doi: 10.1152/ajpcell.00239.2017. Epub 2018 May 23.


Kutzner TJ, Higuero AM, Süßmair M, Kopitz J, Hingar M, Díez-Revuelta N, Caballero GG, Kaltner H, Lindner I, Abad-Rodríguez J, Reusch D, Gabius HJ. How presence of a signal peptide affects human galectins-1 and -4: Clues to explain common absence of a leader sequence among adhesion/growth-regulatory galectins. Biochim Biophys Acta Gen Subj. 2020 Jan;1864(1):129449.


Oide S, Tanaka Y, Watanabe A, Inui M. Carbohydrate-binding property of a cell wall integrity and stress response component (WSC) domain of an alcohol oxidase from the rice blast pathogen Pyricularia oryzae. Enzyme Microbial Technology. 2019 Jun;125:13-20, doi:10.1016/j.enzmictec.2019.02.009. Epub 2019 Feb 25.

Cites the use of PNGase F PRIME™ in TritonX-100 nonionic detergent.


Everest-Dass AV, Moh ESX, Ashwood C, Shathili AMM, Packer NH. Human Disease Glycomics: Technology Advances Enabling Protein Glycosylation Analysis - Part 1. Expert Rev Proteomics: 2018 Feb;15(2):165-182. doi: 10.1080/14789450.2018.1421946. Epub 2018 Jan 11.


Holst S, van Pelt GW, Mesker WE, Tollenaar RA, Belo AI, van Die I, Rombouts Y, Wuhrer M. High-Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells. Methods Mol Biol. 2017;1503:185-196.


Sonneveld ME, Natunen S, Sainio S, Koeleman CA, Holst S, Dekkers G, Koelewijn J, Partanen J, van der Schoot CE, Wuhrer M, Vidarsson G. Glycosylation pattern of anti-platelet IgG is stable during pregnancy and predicts clinical outcome in Alloimmune Thrombocytopenia. Br J Haematol. 2016 Jul;174(2):310-20. doi: 10.1111/bjh.14053. Epub 2016 Mar 28.


Holst S, Deuss AJ, van Pelt GW, van Vliet SJ, Garcia-Vallejo JJ, Koeleman CA, Deelder AM, Mesker WE, Tollenaar RA, Rombouts Y, Wuhrer M. N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression. Mol Cell Proteomics. 2016 Jan;15(1):124-40. doi: 10.1074/mcp.M115.051235. Epub 2015 Nov 


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