Endo F3 PRIME-LY™
| Target Core | Cleaves in β(1-4) link between the two core GlcNAcs of asparagine linked glycans. Endo F3 PRIME-LY™ cleaves this link on core-fucosylated structures. |
|---|---|
| Compatible Glycoprotein | Native and denatured |
| recombinant, expressed in E. Coli. | |
| Total Protocol Length | 30 minutes to 24 hours |
| Typical Yield | 1 µg of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. |
| Quantity | Each standard vial of lyophilized format enzyme contains 100µg of Endo F3 PRIME™ lyophilized from 20mM Tris-HCl, 50mM NaCl, pH 7.5. The final concentration of the enzyme is ultimately determined by the amount of cell culture grade water used in the reconstitution process. |
| Purity | ≥95% as determined by SDS PAGE |
| Contains Glycerol | No |
| Compatible with Mass Spec | Yes |
| Affinity tag for purification | Yes. Endo F3 PRIME contains a His-tag. |
| Reaction Conditions | In vitro and in situ |
| Storage | -20°C or 4°C (avoid multiple freeze-thaw cycles.) |
| Shelf Life | 1 year |